Journal: Hepatology Communications

Article Title: Profiling of cell‐free DNA methylation and histone signatures in pediatric NAFLD: A pilot study

doi: 10.1002/hep4.2082

Figure Lengend Snippet: Comparison of characteristics in children without (no NASH) versus children with NASH

Article Snippet: The patatin‐like phospholipase domain containing 3 (PNPLA3) rs738409, transmembrane 6 superfamily member 2 (TM6SF2) rs58542926, membrane bound O‐acyltransferase domain containing 7 (MBOAT7) rs641738, and klotho‐β (KLB) rs17618244 variants were genotyped by allelic discrimination using TaqMan 5′‐nuclease assays (Life Technologies).

Techniques: Comparison

Journal: Talanta Open

Article Title: An alternative ready-to-use electrochemical immunosensor for point-of-care COVID-19 diagnosis using graphene screen-printed electrodes coupled with a 3D-printed portable potentiostat

doi: 10.1016/j.talo.2022.100155

Figure Lengend Snippet: % Relative response with respect to negative samples of the developed immunosensor for SARS-CoV-2 RBD protein compared against Middle East Respiratory Syndrome Coronavirus (MERS-CoV) as well as Alpha, Beta, and Gamma variations of SARS-CoV-2 at 10 pg mL −1 .

Article Snippet: SARS-CoV-2 Spike Trimer P.1, Gamma variant (cat no.: 100989-1, purity ≥ 90%) and SARS-CoV-2 Spike Trimer B.1.351, Beta variant (cat no.: 101091, purity ≥ 90%) were procured from BPS Bioscience (San Diego, USA).

Techniques:

Journal: International Journal of Oncology

Article Title: HAF drives the switch of HIF-1α to HIF-2α by activating the NF-κB pathway, leading to malignant behavior of T24 bladder cancer cells

doi: 10.3892/ijo.2013.2210

Figure Lengend Snippet: The antibodies used.

Article Snippet: For understanding the roles of HIFs, pcDNA3.1-hif-1α and pcDNA3.1-hif-2α (Addgene plasmid 18949 and 18950, http://www.addgene.org ) were cotransfected with pCMV-hif-β (pCMV-HIF-β-hygro, HG13010-M, Sino Biological Inc. China) into the T24 cell, respectively.

Techniques:

Journal: International Journal of Oncology

Article Title: HAF drives the switch of HIF-1α to HIF-2α by activating the NF-κB pathway, leading to malignant behavior of T24 bladder cancer cells

doi: 10.3892/ijo.2013.2210

Figure Lengend Snippet: Primers for real-time PCR and siRNA sequence.

Article Snippet: For understanding the roles of HIFs, pcDNA3.1-hif-1α and pcDNA3.1-hif-2α (Addgene plasmid 18949 and 18950, http://www.addgene.org ) were cotransfected with pCMV-hif-β (pCMV-HIF-β-hygro, HG13010-M, Sino Biological Inc. China) into the T24 cell, respectively.

Techniques: Real-time Polymerase Chain Reaction, Sequencing

Journal: International Journal of Oncology

Article Title: HAF drives the switch of HIF-1α to HIF-2α by activating the NF-κB pathway, leading to malignant behavior of T24 bladder cancer cells

doi: 10.3892/ijo.2013.2210

Figure Lengend Snippet: Hypoxia induces the enhanced malignancy and activation of NF-κB in T24 cells, resulting in the elevation of EMT related genes and HIFs. (A) Western blotting showing 48-h hypoxia induces the upregulation of MMP 2 , MMP 9 , N-cadherin, vimentin, Slug and Zeb1 but downregulation of E-cadherin. (B) Statistical chart of the Boyden chamber assay suggests that the ability of migration/invasion is significantly enhanced after 48-h hypoxic exposure. (C) The representative figures of Boyden chamber assay. (D) Western blotting showing the increased HIF-2α, HIF-β and HAF but decreased HIF-1α by prolonged hypoxia exposure. (E) Real-time PCR shows that the prolonged hypoxia exposure induces the elevation of hifs and haf , but has no effect on the iκb and p65 . (F) Western blotting indicating the nuclear accumulation of P65 induced by hypoxia.

Article Snippet: For understanding the roles of HIFs, pcDNA3.1-hif-1α and pcDNA3.1-hif-2α (Addgene plasmid 18949 and 18950, http://www.addgene.org ) were cotransfected with pCMV-hif-β (pCMV-HIF-β-hygro, HG13010-M, Sino Biological Inc. China) into the T24 cell, respectively.

Techniques: Activation Assay, Western Blot, Boyden Chamber Assay, Migration, Real-time Polymerase Chain Reaction

Journal: International Journal of Oncology

Article Title: HAF drives the switch of HIF-1α to HIF-2α by activating the NF-κB pathway, leading to malignant behavior of T24 bladder cancer cells

doi: 10.3892/ijo.2013.2210

Figure Lengend Snippet: Hypoxia induces the nuclear translocation of NF-κB and the degradation of IκB/p-IκB, which also occurred in the T24 haf cells, resulting in the EMT and elevation of NF-κB. (A) Western blotting showing HAF induces the upregulation of N-cadherin, vimentin, Slug, Zeb1, MMP 2 , MMP 9 and T-P65, but downregulation of E-cadherin and HIF-1α, accompanied by the elevation of HIF-2α, HIF-β. (B) Immunofluorescence of the nuclear translocation of P65 induced by prolonged hypoxia. (C) Western blotting indicating the elevated p-IκB in T24-HAF cells in the presence of PDTC. (D) Western blotting showing the HAF-overexpression contributing to the elevation of p-IκB but without visible change of IκB. (E) Western blotting indicating the prolonged term hypoxia induces the elevation of p-IκB but without visible change of IκB. (F) Western blotting of the HAF-overexpression induces the accumulation of P65 in the nuclear.

Article Snippet: For understanding the roles of HIFs, pcDNA3.1-hif-1α and pcDNA3.1-hif-2α (Addgene plasmid 18949 and 18950, http://www.addgene.org ) were cotransfected with pCMV-hif-β (pCMV-HIF-β-hygro, HG13010-M, Sino Biological Inc. China) into the T24 cell, respectively.

Techniques: Translocation Assay, Western Blot, Immunofluorescence, Over Expression

Journal: International Journal of Oncology

Article Title: HAF drives the switch of HIF-1α to HIF-2α by activating the NF-κB pathway, leading to malignant behavior of T24 bladder cancer cells

doi: 10.3892/ijo.2013.2210

Figure Lengend Snippet: HAF overexpression-induced malignancy, NF-κB nuclear translocation, EMT and alternation of HIFs in T24 cell can be inhibited by PDTC. (A) Representative images of Boyden chamber assay for the attenuated ability of migration/invasion in T24-HAF cells in the presence of PDTC. (B) Western blotting showing the phenomenon induced by PDTC, manifested as the decreased N-cadherin, vimentin, Zeb1, Slug, MMP 2 , MMP 9 and increased E-cadherin. (C) Real-time PCR indicating that haf contributes to the elevation of hif-1α, hif-2α and hif-β but has no visible effect on p65 and iκB . (D) Immunofluorescence suggesting the nuclear translocation of P65 induced by HAF-overexpression, and its inhibition by PDTC. (E) Western blotting of the elevation of HIF-2α and HIF-β was inhibited by PDTC. (F) Real-time PCR of the HAF-induced elevation of hif-1α, hif-2α and hif-β was inhibited by PDTC, which still has no visible effect on p65 and iκB .

Article Snippet: For understanding the roles of HIFs, pcDNA3.1-hif-1α and pcDNA3.1-hif-2α (Addgene plasmid 18949 and 18950, http://www.addgene.org ) were cotransfected with pCMV-hif-β (pCMV-HIF-β-hygro, HG13010-M, Sino Biological Inc. China) into the T24 cell, respectively.

Techniques: Over Expression, Translocation Assay, Boyden Chamber Assay, Migration, Western Blot, Real-time Polymerase Chain Reaction, Immunofluorescence, Inhibition

Journal: International Journal of Oncology

Article Title: HAF drives the switch of HIF-1α to HIF-2α by activating the NF-κB pathway, leading to malignant behavior of T24 bladder cancer cells

doi: 10.3892/ijo.2013.2210

Figure Lengend Snippet: NF-κB-overexpression induces enhanced malignancy and alternation of HIFs in T24 cell. (A) Western blotting showing that p65 gives rise to the elevation of Zeb1, Slug, N-cadherin, vimentin, MMP 2 and MMP9 but decreased E-cadherin. (B) Western blotting showing that p65 initiates the increase of HIF-1α, HIF-2α and HIF-β, but has no effect on HAF. (C) Real-time PCR showing the elevation of hif-1α, hif-2α and hif-β induced by p65 . (D) Western blotting showing the efficiency of P65 transfection and the GFP-fusion protein. (E and F) Boyden chamber assay showing the enhanced ability of migration/invasion induced by P65.

Article Snippet: For understanding the roles of HIFs, pcDNA3.1-hif-1α and pcDNA3.1-hif-2α (Addgene plasmid 18949 and 18950, http://www.addgene.org ) were cotransfected with pCMV-hif-β (pCMV-HIF-β-hygro, HG13010-M, Sino Biological Inc. China) into the T24 cell, respectively.

Techniques: Over Expression, Western Blot, Real-time Polymerase Chain Reaction, Transfection, Boyden Chamber Assay, Migration

Journal: International Journal of Oncology

Article Title: HAF drives the switch of HIF-1α to HIF-2α by activating the NF-κB pathway, leading to malignant behavior of T24 bladder cancer cells

doi: 10.3892/ijo.2013.2210

Figure Lengend Snippet: Dimer of hif-1α/β and hif-2α/β has different target gene profiles in T24 cell. (A) Western blotting indicating no visible difference of EMT related genes plus stem cell markers induced by HIF-1α/β and Vec/Vec. (B) Western blotting indicating the elevation of Nanog, Oct-4 and EMT-related genes in the T24HIF-2α/β compared with the Vec/Vec. (C and D) Western blotting showing the efficiency of cotransfection of HIF-1α/β or HIF-2α/β. (E) Real-time PCR showing the different target genes in the three subclones. Hif-1α, hif-2α and hif-β are all elevated in T24 hif-1α/β and T24 hif-2α/β . Specifically, hif-1α/β results in the induction of pgk1, ldha and aldoc, hif-2α/β targets nanog and oct-4 . (F) Western blotting showing the discrepancy of targets induced by the two dimers. Clearly, HIF-1α/β affect the metabolism-related genes, but the HIF-2α/β emphasizes the stem-cell related genes.

Article Snippet: For understanding the roles of HIFs, pcDNA3.1-hif-1α and pcDNA3.1-hif-2α (Addgene plasmid 18949 and 18950, http://www.addgene.org ) were cotransfected with pCMV-hif-β (pCMV-HIF-β-hygro, HG13010-M, Sino Biological Inc. China) into the T24 cell, respectively.

Techniques: Western Blot, Cotransfection, Real-time Polymerase Chain Reaction

Journal: International Journal of Oncology

Article Title: HAF drives the switch of HIF-1α to HIF-2α by activating the NF-κB pathway, leading to malignant behavior of T24 bladder cancer cells

doi: 10.3892/ijo.2013.2210

Figure Lengend Snippet: Schematic diagram as summary of the present investigation. Short-term hypoxia leads to the stabilization of HIF-1α, targeting the metabolism-related genes, such as PGK1, ALDOC and LDHA, to adapt to the hypoxic environment. The prolonged hypoxia is more sophisticated. Firstly, this hypoxia induces HAF. On the one hand, the induced HAF contributes to the degradation of IκB in an unknown way, leading to the activation of NF-κB pathway, which can be inhibited by PDTC resulting in the increase of target genes, such as HIF-1α, HIF-2α and HIF-β. On the other hand, HAF leads to the degradation of HIF-1α directly. The combination of the two aspects result in the switch of HIF-1α to HIF-2α, leading to the activation of migration/invasion and stem-cell related genes to maintain invasive and self-renewal capacity of tumor cells.

Article Snippet: For understanding the roles of HIFs, pcDNA3.1-hif-1α and pcDNA3.1-hif-2α (Addgene plasmid 18949 and 18950, http://www.addgene.org ) were cotransfected with pCMV-hif-β (pCMV-HIF-β-hygro, HG13010-M, Sino Biological Inc. China) into the T24 cell, respectively.

Techniques: Activation Assay, Migration